Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
نویسندگان
چکیده
منابع مشابه
Culture of isolated floor plate tissue and production of conditioned medium to assess functional properties of floor plate-released signals.
During development, progenitors and post-mitotic neurons receive signals from adjacent territories that regulate their fate. The floor-plate is a group of glial cells lining the ependymal canal at ventral position. The floor-plate expresses key morphogens contributing to the patterning of cell lineages in the spinal cord. At later developmental stages, the floor-plate regulates the navigation o...
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During early neurogenesis, the floor plate plays an essential role(s) in the differentiation of the ventral portion of neural tube. In this study, we detected the specific distribution of unique glycoconjugate during the floor plate differentiation. Formalin fixed paraffin sections of BALB/c mice (10-14 embryonic days) were processed for histochemical studies using horseradish peroxidase-labell...
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The floor plate is located at the ventral midline of the neural tube and has been implicated in neural cell patterning and axon guidance. To address the cellular mechanisms involved in floor plate differentiation, we have used an assay that monitors the expression of floor-plate-specific antigens in neural plate explants cultured in the presence of inducing tissues. Contact-mediated signals fro...
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in this thesis, at first we investigate the bounded inverse theorem on fuzzy normed linear spaces and study the set of all compact operators on these spaces. then we introduce the notions of fuzzy boundedness and investigate a new norm operators and the relationship between continuity and boundedness. and, we show that the space of all fuzzy bounded operators is complete. finally, we define...
15 صفحه اولunique carbohydrate appearance of the floor plate during early neurolation
during early neurogenesis, the floor plate plays an essential role(s) in the differentiation of the ventral portion of neural tube. in this study, we detected the specific distribution of unique glycoconjugate during the floor plate differentiation. formalin fixed paraffin sections of balb/c mice (10-14 embryonic days) were processed for histochemical studies using horseradish peroxidase-labell...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/50884